Fig. 1.

Proteolytic cleavage of the individual N-linked glycosylation motif mutants. A Schematic diagram and cartoon representation (PDB: 5VK2 (1)) of the glycosylation of LASV GPC. The precursor glycoprotein GPC was cleaved by SPase and SKI-1/S1P. The mature GPC contains SSP (1–58), GP1 (59–259), and GP2 (260–491) domains and is

modified by 11 N-linked glycan chains (green). Transmembrane domains are indicated in gray. B–D HEK 293 T cells expressing the wild-type GPC and individual N-glycosylation mutants of N → Q (B), N → A (C), and S/T → A (D), respectively. The expressed proteins were separated via SDS-PAGE, and western blotting was carried out using anti-GP2 antisera. Images chosen are representative of at least three independent assays.