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. 2021 Mar 10;36(4):774–783. doi: 10.1007/s12250-021-00358-y

Fig. 3.

Fig. 3

Effects of N-glycan deletion on T cells in spleen lymphocytes. The mouse spleen lymphocytes were separated, cultured, and stained with phycoerythrin (PE)-conjugated rat anti-mouse CD8a, fluorescein isothiocyanate-conjugated rat anti-mouse CD4, PE-CyTM7-conjugated hamster anti-mouse CD3e, and 7-AAD viability staining solution (BioLegend) at 4 °C for 20 min in the dark. A–C Effects of individual N-glycosylation deletions on CD3+ (A), CD4+ (B), and CD8+ (C) T cells in spleen lymphocytes. Effector T cells CD4+ (D) and CD8+ (E) T cells were stained with allophycocyanin-conjugated rat anti-mouse IFN-y antibody (1:200). Each group contained five to six mice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared to WT.