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Effects of N-glycan deletions on cytokines secreted by spleen lymphocytes were labeled by using a cytometric bead array kit (BD Biosciences), and IL-2 (A), IFN-γ (B), TNF-α (C), IL-4 (D), IL-6 (E), IL-10 (F), and IL-17A (G) were determined using flow cytometry. Each group contained five to six mice. *P < 0.05, **P < 0.01, ***P < 0.001, compared to WT.
