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. 2020 Nov 30;31(4):1927–1952. doi: 10.1093/cercor/bhaa331

Figure 2.

Figure 2

FIB/SEM imaging in the stratum radiatum of the rat CA1. (A) Low-magnification photomicrograph of a coronal Nissl-stained brain section containing the hippocampus (CA1 and CA3: Cornu ammonis 1 and 3, GD: dentate gyrus). (B) Higher magnification of the CA1 regions showing the layers (Or: stratum oriens, Pyr: stratum pyramidale, Rad: stratum radiatum, Lac-Mol: stratum lacunosum-moleculare). (C) Image taken with the SEM from the surface of the block containing the hippocampus to be analyzed. Rectangle surrounds the region shown in D. (D) Image taken with the SEM showing—at higher magnification—the stratum pyramidale and the trenches (arrowheads) opened with the FIB in the stratum radiatum to acquire the FIB/SEM serial images. Arrows indicate the direction of the FIB/SEM imaging. Scale bar in D corresponds to: 1 mm in (A) 412 μm in (B) 625 μm in (C) 100 μm in D.