Fig. 4.

NSC self-renewal was promoted by FoxM1-activated miR-335-3p. NSCs were treated with oe-NC, oe-FoxM1, si-NC, si-FoxM1, oe-FoxM1 + NC-inhibitor, and oe-FoxM1 + miR-335-3p inhibitor. a miR-335-3p expression in NSCs detected by RT-qPCR. b Statistical analysis of the number and size of neurospheres. c NSC cell cycle measured by flow cytometry. d Expression of self-renewal-related and differentiation-related genes in NSCs examined by RT-qPCR. The measurement data were expressed as mean ± standard deviation. Data analyses among multiple groups were performed by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05 versus oe-NC or si-NC or oe-FoxM1 + NC-inhibitor. ^#p < 0.05 versus oe-FoxM1 + NC-inhibitor and oe-NC. The experiment was performed three times