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. 2021 Mar 9;22:171. doi: 10.1186/s12864-021-07473-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The SNP genotype results and the difference of lotus varieties rhizome traits between three groups with different SNP alterations. a SNP genotyping of target single nucleotide. KASP assay showing clustering of lotus varieties on the X-axe (Allele 1 with FAM) and Y-axe (Allele 2 with HEX). RFU is the Relative Fluorescence Units. Red dots represent varieties have the FAM-type allele 1; green dots represent varieties have the HEX-type allele 2; blue dot represents the NTC (non-template control). b, c, and d: Boxplot of Perimeter, REI, and sugar content in group I (SNP genotype: CC), group II (SNP genotype: TT), and group III (SNP genotype: C/T), respectively. The straight line represents median, the dotted line shows mean value and open circles indicate outliers. Upper and lower lines indicate maximum and minimum values. *, P < 0.05; **, P < 0.01. ns, not significant