. 2021 Mar 10;12:28. doi: 10.1186/s40104-021-00552-8

Table 3.

Primer and probe sequences used for real-time PCR

Items	Primer and probe sequence (5´→3´)	Product length, bp
Total bacteria	F: ACTCCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG	200
Lactobacillus	F: GAGGCAGCAGTAGGGAATCTTC R: CAACAGTTACTCTGACACCCGTTCTTC	126
Lactobacillus	P: AAGAAGGGTTTCGGCTCGTAAAACTCTGTT
Bifidobacterium	F: CGCGTCCGGTGTGAAAG R: CTTCCCGATATCTACACATTCCA	121
Bifidobacterium	P: ATTCCACCGTTACACCGGGAA
Bacillus	F: GCAACGAGCGCAACCCTTGA R: TCATCCCCACCTTCCTCCGGT	92
Bacillus	P: CGGTTTGTCACCGGCAGTCACCT
Escherichia coli	F: CATGCCGCGTGTATGAAGAA R: CGGGTAACGTCAATGAGCAAA	96
Escherichia coli	P: AGGTATTAACTTTACTCCCTTCCTC