Fig. 4.

The RBD-SD1 probe interacts with human primary bone marrow cells and SARS-CoV-2 induces a sharp increase of ACE2 mRNA expression in human primary bone marrow cells. (A) Representative histograms of flow cytometry analysis to determine RBD-SD1 probe labeling of human bone marrow cells from donor 1. The dashed line indicates the gate between the RBD-SD1 probe-negative and -positive cells. (B) Representative histograms of flow cytometry analysis to determine RBD-SD1 probe labeling of human bone marrow cells from donor 2. The dashed line indicates the gate between the RBD-SD1 probe-negative and -positive cells. (C) RT-qPCR of ACE2 in primary human bone marrow cells infected with the indicated amount (MOI) of SARS-CoV-2 for 3 days (n = 3 biological replicates, error bars indicate SEM). For all panels, ^**p ≤ 0.01, and ^***p ≤ 0.001.