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. 2021 Feb 24;12:568197. doi: 10.3389/fendo.2021.568197

Figure 4.

Figure 4

Molecular processes changed in the heart muscle of obese rats (n=5). A scaling plot of individual proteomes revealed the relatively homogenous composition in the control group (ContO) and the more dispersed proteomic phenotype in experimental (obese) animals (A).Two biological processes: (B) the directed movement of a phospholipid out of a cell or organelle (p=0.024), and (C) the chemical reactions and pathways involving ATP (p=0.043), were significantly changed in the tissue (Bonferoni correction). By using the INTACT database we determined that the pool of all significantly changed proteins interacted with GLUT-4 (27 proteins, p<0.001, Fisher correction) and ACSL-1 (four proteins, p=0.0035, Fisher correction), suggesting that these proteins may be impacted by obesity. The evaluation of the protein expression by means of WB showed that the cardiac expression of ACSL-1 (D) (p=0.045) but not GLUT-4 (E) was changed (increased) in obese rats. GLUT-1 is the next glucose carrier in cardiomyocytes beyond GLUT-4, and its expression was downregulated in obese rats (F) (p=0.026). The Student’s t-test was used for analyzing the data (*p<0.05, ***p<0.001). ACSL-1, long-chain-fatty-acid-CoA ligase 1; AK1, adenylate kinase isoenzyme 1; ATP5L, ATP synthase subunit; GLUT-1, solute carrier family 2, facilitated glucose transporter member 1; GLUT-4, solute carrier family 2, facilitated glucose transporter member 4; HSP70-1, heat shock 70 kDa protein 1A.