Skip to main content
. 2021 Feb 18;9:637565. doi: 10.3389/fcell.2021.637565

FIGURE 2.

FIGURE 2

GFP–SNAP29 partially colocalizes with endoplasmic reticulum (ER) and Golgi apparatus (GA) markers. (A–I) Single sections of HeLa cells over-expressing GFP–SNAP29 for 6 h stained as indicated and acquired by stimulated emission depletion microscopy. The dashed and the continuous lines delimit the nucleus and the plasma membrane, respectively. The boxed GA area is magnified in the insets. Yellow arrows point to an example of co-localization between GFP–SNAP29 and ER or GA markers. (J,K) Cryo-immuno-EM sections of HeLa cells stably transfected to express GFP–SNAP29, stained, and revealed as indicated. Some GFP–SNAP29 localize to the ERGIC area and colocalizes with a COPI marker.