FIGURE 3.

SNAP29 contributes to ManII–SBP–GFP trafficking to the Golgi apparatus (GA). (A–E) Single confocal sections of HeLa cells stably expressing ManII–SBP–GFP, treated and stained as indicated. The EGFP pattern has been imaged before addition of biotin (no biotin) and 20 min after addition of biotin (20 min biotin). The insets show close-ups of the GA and surrounding areas. (F) Quantification of the ratio of the Giantin-positive EGFP signal over total, relative to the experiment in panel (A–E). SNAP29, as well as the endoplasmic reticulum and GA SNAREs STX18, STX5, and SEC22B, appears to support ManII–SBP–GFP trafficking to the GA. The median with interquartile range is shown, and the p-value is obtained by Dunn’s multiple-comparisons test.