Table 2. Comparison of the antibody pairs and detection limit between the original and the modified stNS1-ELISA.
| Dengue serotypes | Original ELISA | Modified ELISA | ||
|---|---|---|---|---|
| Antibody pairs (Capture Ab/ detection Ab) | Detection limit (ng/mL)# | Antibody pairs (Capture Ab/ detection Ab*) | Detection limit (ng/mL)# | |
| DENV1 | 2E11/84B | 4.4 | 2E11/84B* | 0.20 |
| DENV2 | 2E11/3D1 | 15.8 | 2E11/1B10* | 0.02 |
| DENV3 | 2E11/46A | 2.9 | 2E11/46A* | 0.02 |
| DENV4 | 2E11/4A | 1.7 | 2E11/4A* | 0.05 |
* Biotinylated antibody.
# Obtained from the lowest NS1 concentration which gave the OD reading above the cut-off value (mean OD reading of negative control antigen (4% BSA-PBS or mock culture sup) + 2 standard deviations (SD).