Table 3. Performance evaluation of the modified stNS1-ELISA vs. RT-PCR in clinical specimens.
| Clinical samples diagnosed by RT-PCR | Modified stNS1-ELISA | |||||
|---|---|---|---|---|---|---|
| Numbers of positive samples | Numbers of negative samples | |||||
| 2E11/84B* DENV1 | 2E11/1B10* DENV2 | 2E11/46A* DENV3 | 2E11/4A* DENV4 | Total | ||
| DENV1 (n = 50) | 31 | 0 | 0 | 0 | 31 | 19 |
| DENV2 (n = 55) | 0 | 46 | 0 | 0 | 46 | 9 |
| DENV3 (n = 45) | 0 | 0 | 38 | 0 | 38 | 7 |
| DENV4 (n = 50) | 0 | 0 | 0 | 41 | 41 | 9 |
| Total (n = 200) | 31 | 46 | 38 | 41 | 156 | 44 |
| OFI (n = 50) | 0 | 0 | 0 | 0 | 0 | 50 |
| Grand total (n = 250) | 31 | 46 | 38 | 41 | 156 | 94 |
| % Serotype agreement to PCR | 100% (31/31) | 100% (46/46) | 100% (38/38) | 100% (41/41) | ||
| % Sensitivity to each serotype | 62.0% (31/50) | 83.6% (46/55) | 84.4% (38/45) | 82.0% (41/50) | ||
| % Overall sensitivity | 78% (156/200) | |||||
| % Overall specificity | 100% (50/50) | |||||
| % Accuracy for dengue diagnosis | 82.4% (206/250) | |||||