Table 4. Performance evaluation of the modified stNS1-ELISA (plus) vs. RT-PCR in clinical specimens.
| Clinical samples diagnosed by RT-PCR | Modified stNS1-ELISA (plus) | ||||||
|---|---|---|---|---|---|---|---|
| Numbers of positive samples | Numbers of negative samples | ||||||
| 2E11/84B* DENV1 | 2E11/1B10* DENV2 | 2E11/46A* DENV3 | 2E11/4A* DENV4 | 2E11/5F3* DENV1/3 | Total | ||
| DENV1 (n = 50) | 31 | 0 | 0 | 0 | 14 | 45 | 5 |
| DENV2 (n = 55) | 0 | 46 | 0 | 0 | ND# | 46 | 9 |
| DENV3 (n = 45) | 0 | 0 | 38 | 0 | 1 | 39 | 6 |
| DENV4 (n = 50) | 0 | 0 | 0 | 41 | ND# | 41 | 9 |
| Total (n = 200) | 31 | 46 | 38 | 41 | 15 | 171 | 29 |
| OFI (n = 50) | 0 | 0 | 0 | 0 | ND# | 0 | 50 |
| Grand total (n = 250) | 31 | 46 | 38 | 41 | 15 | 171 | 79 |
| % Serotype agreement to PCR | 100% (31/31) | 100% (46/46) | 100% (38/38) | 100% (41/41) | |||
| % Sensitivity to each serotype* | 90.0% (45/50) | 83.6% (46/55) | 86.7% (39/45) | 82.0% (41/50) | |||
| % Overall sensitivity | 85.5% (171/200) | ||||||
| % Overall specificity | 100% (50/50) | ||||||
| % Accuracy for dengue diagnosis | 88.4% (221/250) | ||||||
ND# = not done.