Fig 3. Less production of Th1/Th17 cytokine and elevated Treg response in spleen and lung of SND1^-/- mice compared with that of control mice following Cm lung infection.

Spleen and lung collected at day 7 p.i. were analyzed for cytokine production by intracellular cytokine staining, as described in Materials and Methods. (A and E) IFN-γ-producing CD8⁺ T cells. (B and F) IFN-γ-producing CD4⁺ T cells.(C and G) IL-17-producing CD4⁺ T cells. (D and H) Foxp3⁺ Treg cells. Summary graphs represent IFN-γ production by CD8⁺ and CD4⁺ T cells and IL-17 by CD4⁺ T cells and Treg cells, respectively, in each group. (I and J) Total cytokine-producing T cells numbers in spleen and lung. Data are presented as the mean±SD of per each group. At least three independent experiments with four mice in each group were performed, and one representative experiment is shown. *, p <0.05; **, p<0.01. + indicates as Cm infection.