Table 1. Neutralization titers in 293T cells with low, medium, and high levels of ACE2 and TMPRSS2.
| Target cells (level) | Neutralization titer IC50 or ID50 (SD) | Neutralization titer IC80 or ID80 (SD) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rabbit anti-RBD | Rabbit anti-S1 | NIBSC plasma | 10G6H5 (μg/ml) | NHS | Rabbit anti-RBD | Rabbit anti-S1 | NIBSC plasma | 10G6H5 (μg/ml) | NHS | |
| 293T-ACE2s | 9377 (16) | 6742 (2765) | 3008 (651) | 0.119 (0.025) | <40 | 3065 (22) | 2053 (668) | 789 (96) | 0.467 (0.079) | <40 |
| 293T-ACE2. TMPRSS2t (low) | 9527 (87) | 5663 (1302) | 3130 (601) | 0.166 (0.051) | <40 | 2915 (92) | 1928 (179) | 737 (93) | 0.478 (0.153) | <40 |
| 293T-ACE2. TMPRSS2t (med) | 9542 (636) | 5462 (765) | 2893 (641) | 0.197 (0.038) | <40 | 2903 (71) | 1936 (135) | 708 (74) | 0.557 (0.111) | <40 |
| 293T-ACE2. TMPRSS2t (high) | 10540 (1508) | 6652 (1649) | 2355 (926) | 0.163 (0.051) | <40 | 3271 (55) | 2339 (515) | 636 (257) | 0.590 (0.112) | <40 |
NHS: Negative control human sera, SD: Standard deviation.