Fig 5. ZBTB2 deletion enhances HIV-1 gene expression and viral replication of VPR knockout strains.

(A) WT or ΔZBTB2 Jurkat and SupT1 cells were challenged with equivalent amounts (based on p24 levels) of either VSV-G pseudotyped HIV-1 vector with nonsense mutations in envelope, luciferase in the NEF locus, and either a WT Vpr or a Vpr nonsense mutant (ΔVpr). (B) Replication of WT or ΔVpr HIV-1 in WT or ΔZBTB2 Jurkat cells. Cells (1X10⁶) were infected with the indicated virus at an moi of 0.005. Supernatant was titered in quadruplicate on TZMBL reporter cells at the indicated time points and infectious units counted by X-gal staining. The data shown are the average mean values obtained in an experiment performed with quadruplicate samples and are representative of three independent experiments. The bar graph is a focus on the day 11 time point. Error bars indicate the standard deviation of the data in all panels. ANOVA analysis was performed and for P-values < 0.05 a Tukey’s HSD was performed and relevant P-values reported.