Fig 12. Activation of the Vpr/ATR pathway impairs ZBTB2 recruitment to the HIV-1 promoter.

(A) ChIP analysis of ZBTB2 recruitment to the HIV-1 promoter in Jurkat cells infected with an HIV-1 NL43 variant that either expresses Vpr or lack Vpr. (B) ChIP analysis of ZBTB2 recruitment to the HIV-1 promoter in Jurkat cells treated with 8 μM methyl methanesulfonate (MMS) (C) 25 mM Hydroxyurea or (D) 4μg/ml ICR-191 and infected with an HIV-1 vector. (E) ChIP analysis of ZBTB2 recruitment to the HIV-1 promoter in primary PBMCs stimulated with CD3/CD28 beads and infected with an NL43 variants either expresses or lacks Vpr. Immunoprecipitation and Real-time PCR analysis were performed and normalized to input controls and reported as percent of starting material for immunoprecipitation. Fold enrichment of experimental IPs relative to IgG controls is reported below the graph. The data shown are the average mean values obtained in an experiment performed with quadruplicate samples and are representative of three independent experiments. Error bars indicate the standard deviation of the data in all panels. P-values were calculated using a standard Student’s t-test and significant changes relative to relevant bracketed comparisons indicated.