TABLE 3.

Summary of Selectivity for MAGE-A3 CARs and TCRs Using Jurkat/T2 Assay and Primary T-cell Assays

The selectivity window is the ratio of EC50s or EC_min of on-target peptide over off-target peptide (eg, MAGE-A12:A3 or EPS8L2:A3). EC_min is the minimum peptide concentration with statistically significant difference from baseline.

(A) MAGE-A3_112–120 TCR and CAR in Jurkat/T2 assays; (B) MAGE-A3_271–279 CAR and TCR selectivity in T2/Jurkat cell assays; (C) Selectivity of MAGE-A3_112–120 CAR and TCR in primary T-cell assays with MCF7 or HEK293 cells loaded with peptide. For readout/assay details, see the Materials and methods section.

CAR indicates chimeric antigen receptors; NA, not applicable; ND, not determined; TCR, T-cell receptor.