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. 2021 Mar 10;7(11):eabd3994. doi: 10.1126/sciadv.abd3994

Fig. 2. GI motility is slowed in the mouse model of medullary α-synucleinopathy.

Fig. 2

(A) Mice inoculated with AAV-A53T, but not with AAV-EV, exhibited slowed GI motility at 6, but not 3, weeks after inoculation [one-way analysis of variance (ANOVA), F3,22 = 5.66, P = 5 × 10−3]. (B) Injection of AAVs harboring Cre-dependent hM4Di DREADDs into the cervical vagus of ChAT-Cre mice leads to selective transfection of cholinergic motoneurons in the DMV. Intraperitoneal injection of the DREADD ligand, CNO, but not vehicle (saline), slowed GI motility (one-way ANOVA, F2,17 = 12.76, P = 4 × 10−4), indicating that silencing these neurons suffices to recapitulate the effect of AAV-A53T transfection. Injection of CNO had no effect on GI motility in noninjected mice (one-way ANOVA, F2,6 = 2.51, P = 0.16). RST, two-tailed Wilcoxon rank sum test; SRT, two-tailed Wilcoxon signed-rank test.