Table 1.
Peptide-specific transitions and voltage parameters for mass spectrometric detection
| Analyte | Transition (m/z) | Dwell time (ms) | Declustering potential (V) | Entrance potential (V) | Collision energy (V) | Collision cell exit potential (V) |
|---|---|---|---|---|---|---|
| Kallidin | 396.9➔506.3 | 65 | 95 | 9 | 23 | 14 |
| Bradykinin | 530.9➔522.4 | 65 | 120 | 10 | 31 | 14 |
| Des-Arg(9)-bradykinin | 452.8➔263.2 | 75 | 85 | 10 | 22 | 15 |
| Des-Arg(10)-kallidin | 516.8➔752.5 | 65 | 100 | 10 | 29 | 11 |
| Bradykinin 2-9 | 452.8➔404.3 | 50 | 120 | 9 | 24 | 11 |
| Bradykinin 1-7 | 379.3➔642.4 | 75 | 63 | 10 | 16 | 10 |
| Bradykinin 1-5 | 287.2➔408.3 | 50 | 61 | 8 | 15 | 11 |
| [Phe8Ψ(CH-NH)-Arg9]-bradykinin | 523.9➔274.3 | 75 | 100 | 12 | 48 | 18 |
ms milliseconds, m/z mass-to-charge ratio, V volt