Table 3.

Absolute matrix effect, CV of internal standard normalised matrix factor of 6 human sources and recovery for all kinin peptides

Analyte	Absolute matrix effect
	QC high (750.0 pg/mL)	QC mid (125.0 pg/mL)	QC low (31.1 pg/mL)	QC low (7.8 pg/mL)
Bradykinin 1-5	−54.3%	−54.9%	−52.9%	-
Kallidin	−25.5%	−27.6%	−27.5%	−25.6%
Bradykinin 1-7	−27.1%	−30.7%	−29.5%	−22.0%
Bradykinin	−19.0%	−11.4%	−13.4%	−11.2%
Des-Arg(10)-kallidin	−20.7%	−16.9%	−14.2%	−17.3%
Bradykinin 2-9	−31.3%	−24.8%	−25.7%	-
Des-Arg(9)-bradykinin	−26.3%	−28.0%	−27.3%	−16.1%
Analyte	Internal standard normalised matrix factor (CV, n = 6)
	QC high (750.0 pg/mL)		QC low (31.1 pg/mL)
Bradykinin 1-5	11.2%		10.3%
Kallidin	1.9%		3.6%
Bradykinin 1-7	0.8%		3.5%
Bradykinin	2.1%		4.8%
Des-Arg(10)-kallidin	2.0%		2.5%
Bradykinin 2-9	1.5%		6.6%
Des-Arg(9)-bradykinin	1.0%		2.7%
Analyte	Recovery
	QC high (750.0 pg/mL)	QC mid (125.0 pg/mL)	QC low (31.1 pg/mL)	QC low (7.8 pg/mL)
Bradykinin 1-5	73.3%	60.9%	74.3%	-
Kallidin	90.8%	87.0%	79.4%	95.7%
Bradykinin 1-7	80.2%	74.6%	82.0%	78.0%
Bradykinin	93.2%	87.9%	91.0%	92.1%
Des-Arg(10)-kallidin	93.8%	91.7%	91.4%	105.2%
Bradykinin 2-9	95.5%	88.3%	92.4%	-
Des-Arg(9)-bradykinin	97.7%	98.8%	93.9%	98.5%

CV coefficient of variation, QC quality control