Table 4.

Parallelism of the kinin peptides. Values of endogenous/spiked plasma samples as stock concentration and back-calculated values of diluted samples

Analyte		Source 1	Source 2	Source 3
Kallidin	Undiluted concentration	161.9 pg/mL	139.1 pg/mL	155.6 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	162.2	129.5	164.9
	4	157.0	134.2	163.5
	8	157.5	157.7	171.1
	16	137.9	174.9	151.9
	CV (%)	6.5	12.8	3.9
Bradykinin	Undiluted concentration	733.7 pg/mL	63.9 pg/mL	294.7 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	712.9	59.7	305.5
	4	697.9	72.2	315.3
	8	691.0	75.0	325.8
	16	700.9	85.9	323.8
	CV (%)	2.4	14.3	4.0
Des-Arg(10)-kallidin	Undiluted concentration	230.4 pg/mL	214.9 pg/mL	208.4 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	229.1	199.6	229.6
	4	228.1	201.8	212.1
	8	225.8	238.7	222.9
	16	220.5	204.3	236.6
	CV (%)	1.7	7.6	6.1
Des-Arg(9)-bradykinin	Undiluted concentration	812.2 pg/mL	39.5 pg/mL	138.2 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	852.1	39.5	137.6
	4	829.7	44.5	138.3
	8	849.9	47.8	130.6
	16	892.5	< LLOQ	118.7
	CV (%)	3.5	9.5	7.3
Bradykinin2-9	Undiluted concentration	114.9 pg/mL	9.4 pg/mL	66.7 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	103.4	< LLOQ	58.0
	4	130.4	< LLOQ	71.2
	8	119.1	< LLOQ	53.3
	16	104.8	< LLOQ	< LLOQ
	CV (%)	9.7	n/a	10.8
Bradykinin 1-7	Undiluted concentration	196.9 pg/mL	7.3 pg/mL	69.6 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	190.0	3.8	72.2
	4	185.3	2.0	67.6
	8	177.8	< LLOQ	69.9
	16	176.1	< LLOQ	74.8
	CV (%)	4.6	4.8	4.4
Bradykinin 1-5	Undiluted concentration	28.8 pg/mL	< LLOQ	25.4 pg/mL
	Dilution factor	Back-calculated concentration (pg/mL)
	2	34.1	< LLOQ	< LLOQ
	4	< LLOQ	< LLOQ	< LLOQ
	8	< LLOQ	< LLOQ	< LLOQ
	16	< LLOQ	< LLOQ	< LLOQ
	CV (%)	12.0	n/a	n/a

CV coefficient of variation, LLOQ lower limit of quantification, n/a not applicable