Table 5.

Results of the stability assessments

Analyte	Nominal concentration (pg/mL)		Benchtop stability		Freeze-thaw stability			Long-term stability
			1.5 h at 21 °C RE (%)	3 h at 21 °C RE (%)	1 cycle RE (%)	2 cycles RE (%)	4 cycles RE (%)	4 weeks RE (%)
Kallidin	QC high	750.0	−3.3	−9.6	1.1	−7.2	−10.3	−6.7
	QC mid	125.0	−1.7	−10.8	−9.5	−2.1	−2.7	8.6
	QC low	31.1	−4.4	−0.7*	−4.4	1.4	−5.3	6.6
	QC low	7.8	0.0	−0.8	12.4	−14.9	−6.5	14.9
Bradykinin	QC high	750.0	−1.4	−7.4	0.4	−7.8	−8.3	−3.0
	QC mid	125.0	−1.2	1.2	−3.2	−6.2	−10.8	5.9
	QC low	31.1	1.4	8.4*	−1.5	−6.2	−9.8	10.0
	QC low	7.8	4.9	138.0	−1.5	1.6	−7.3	4.5
Des-Arg(10)-kallidin	QC high	750.0	−0.9	−11.2	6.1	−8.4	−7.9	−2.1
	QC mid	125.0	6.9	−2.4	−0.8	−7.7	−7.7	0.9
	QC low	31.1	13.1	−7.9*	−1.2	0.4	−8.6	13.8
	QC low	7.8	9.7	9.4	−11.8	0.8	3.5	10.6
Des-Arg(9)-bradykinin	QC high	750.0	−4.6	−21.8	−0.3	−8.4	−7.9	0.5
	QC mid	125.0	1.1	−16.1	2.1	−7.7	−7.7	3.3
	QC low	31.1	3.4	−5.2*	5.4	0.4	−8.6	12.3
	QC low	7.8	−10.2	11.8	−11.7	0.8	3.5	8.2
Bradykinin 2-9	QC high	750.0	−3.3	−9.1	−1.1	−7.2	−10.6	−0.6
	QC mid	125.0	−2.4	−8.0	−0.5	−12.2	−11.7	1.7
	QC low	31.1	4.7	3.8*	11.8	−4.7	−0.5	9.0
Bradykinin 1-7	QC high	750.0	1.7	2.1	2.0	−6.6	−7.0	−2.6
	QC mid	125.0	9.0	3.6	−1.3	−2.6	−6.1	6.0
	QC low	31.1	3.1	10.7*	4.0	−6.3	−15.3	7.5
	QC low	7.8	11.8	16.0	−7.3	0.3	−12.3	13.6
Bradykinin 1-5	QC high	750.0	−0.6	−7.6	4.0	−8.0	−20.3	−11.0
	QC mid	125.0	−1.1	−2.6	−8.3	−5.8	−14.1	11.3
	QC low	31.1	−8.7	−5.3*	−5.4	−2.8	−30.6	13.9

*n = 1; QC quality control, RE relative error