Video 5. The trajectory of single dI1 axons in the first half of the floor plate (electroporated half) in the absence of Endoglycan was aberrant and showed a 'corkscrew'-like phenotype.

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Example of a tdTomato-positive dI1 axon taken from a 24 hr time-lapse recording of an ‘Endoglycan-knockdown’ spinal cord. Arrowheads show how the growth cone is migrating within the first half (electroporated half) of the floor plate and enters in contact with mislocated EGFP-positive cells (arrows) that induced a 'corkscrew' like morphology of the shaft. The 3D coronal rotation clearly shows that the axon and cells are located within the commissure (arrowhead).