Video 6. The trajectory of single dI1 axons in the first half of the floor plate (electroporated half) in the absence of Endoglycan was aberrant and showed a 'corkscrew'-like phenotype.

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Example of tdTomato-farnesylated-positive dI1 axons taken from 24 hr time-lapse recordings of an ‘Endoglycan-knockdown’ spinal cord. Arrowheads show how two growth cones are migrating within the first half (electroporated half) of the floor plate. The first one made a loop within the floor-plate cell layer (arrowheads), as shown by the 3D coronal rotation. The second one was attracted toward mislocalized EGFP-positive cells (arrow), made contact with them and then carried out a U-turn toward them before continuing its migration in the direction of the midline. The 3D coronal view confirmed that the second growth cone during its U-turn and the mislocalized cells were located within the commissure (arrowhead).