Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | Anti-digoxigenin-AP antibody (rabbit polyclonal) | Roche | RRID:AB_514497 | ISH (1:10000) |
Antibody | Anti-myc (rabbit polyclonal) | Abcam | RRID: AB_307014 | IF (1:500) |
Antibody | Anti-Hnf3β (mouse monoclonal) | DSHB | RRID: AB_2278498 | IF (supernatant) |
Antibody | Anti-Axonin-1 (goat polyclonal) | Stoeckli and Landmesser, 1995 | N/A | IF (1:500) |
Antibody | Anti-Axonin-1 (rabbit polyclonal) | Stoeckli and Landmesser, 1995 | N/A | IF (1:1000) |
Antibody | Anti-neurofilament-M (RMO-270, mouse monoclonal) | ThermoFisher Scientific | RRID: AB_2532998 | IF (1:200) |
Antibody | Anti-GFP-FITC (goat polyclonal) | Rockland | RRID: AB_218187 | IF (1:400) |
Antibody | Anti-NrCAM(mouse monoclonal) | Fitzli et al., 2000 | N/A | IF (1:1000) |
Antibody | Anti-Islet1 (mouse monoclonal) | DSHB | RRID: AB_528315 | IF (supernatant) |
Antibody | Anti-Nkx2.2 (mouse monoclonal) | DSHB | RRID: AB_531794 | IF (supernatant) |
Antibody | Anti-Pax3 (mouse monoclonal) | DSHB | RRID: AB_528426 | IF (supernatant) |
Antibody | Anti-Pax6 (mouse monoclonal) | DSHB | RRID: AB_528427 | IF (supernatant) |
Antibody | Cleaved Caspase-3 (rabbit polyclonal) | Cell Signaling | RRID: AB_2341188 | IF (1:200) |
Cell line (H. sapiens) | HEK293T | American Type Culture Collection | RRID: CVCL_0063 | |
Cell line (H. sapiens) | HEK293T-PODXL2-myc(Endo-myc) | This paper | N/A | |
Recombinant DNA reagent | Math1::chEndoglycan (plasmid) | This paper | N/A | |
Recombinant DNA reagent | Hoxa1::chEndoglycan (plasmid) | This paper | N/A | |
Recombinant DNA reagent | β-actin::chEndoglycan (plasmid) | This paper | N/A | |
Recombinant DNA reagent | β-actin::EGFP-F (plasmid) | This paper | N/A | |
Recombinant DNA reagent | β-actin::mRFP (plasmid) | Wilson and Stoeckli, 2011 | N/A | |
Recombinant DNA reagent | Math1::tdTomato-F (plasmid) | Wilson and Stoeckli, 2011 | N/A | |
Chemical compound, drug | O-glycosidase and Neuraminidase | NEB | Cat# E0540S | |
Software, algorithm | Fiji/ImageJ | Schindelin et al., 2012 | RRID:SCR_002285 | |
Biological sample (Gallus gallus) |
Hubbard JA57 strain | Brüterei Stöckli, Ohmstal | N/A |