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. 2021 Mar 2;10:e64767. doi: 10.7554/eLife.64767

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Antibody Anti-digoxigenin-AP antibody (rabbit polyclonal) Roche RRID:AB_514497 ISH (1:10000)
Antibody Anti-myc (rabbit polyclonal) Abcam RRID: AB_307014 IF (1:500)
Antibody Anti-Hnf3β (mouse monoclonal) DSHB RRID: AB_2278498 IF (supernatant)
Antibody Anti-Axonin-1 (goat polyclonal) Stoeckli and Landmesser, 1995 N/A IF (1:500)
Antibody Anti-Axonin-1 (rabbit polyclonal) Stoeckli and Landmesser, 1995 N/A IF (1:1000)
Antibody Anti-neurofilament-M (RMO-270, mouse monoclonal) ThermoFisher Scientific RRID: AB_2532998 IF (1:200)
Antibody Anti-GFP-FITC (goat polyclonal) Rockland RRID: AB_218187 IF (1:400)
Antibody Anti-NrCAM(mouse monoclonal) Fitzli et al., 2000 N/A IF (1:1000)
Antibody Anti-Islet1 (mouse monoclonal) DSHB RRID: AB_528315 IF (supernatant)
Antibody Anti-Nkx2.2 (mouse monoclonal) DSHB RRID: AB_531794 IF (supernatant)
Antibody Anti-Pax3 (mouse monoclonal) DSHB RRID: AB_528426 IF (supernatant)
Antibody Anti-Pax6 (mouse monoclonal) DSHB RRID: AB_528427 IF (supernatant)
Antibody Cleaved Caspase-3 (rabbit polyclonal) Cell Signaling RRID: AB_2341188 IF (1:200)
Cell line (H. sapiens) HEK293T American Type Culture Collection RRID: CVCL_0063
Cell line (H. sapiens) HEK293T-PODXL2-myc(Endo-myc) This paper N/A
Recombinant DNA reagent Math1::chEndoglycan (plasmid) This paper N/A
Recombinant DNA reagent Hoxa1::chEndoglycan (plasmid) This paper N/A
Recombinant DNA reagent β-actin::chEndoglycan (plasmid) This paper N/A
Recombinant DNA reagent β-actin::EGFP-F (plasmid) This paper N/A
Recombinant DNA reagent β-actin::mRFP (plasmid) Wilson and Stoeckli, 2011 N/A
Recombinant DNA reagent Math1::tdTomato-F (plasmid) Wilson and Stoeckli, 2011 N/A
Chemical compound, drug O-glycosidase and Neuraminidase NEB Cat# E0540S
Software, algorithm Fiji/ImageJ Schindelin et al., 2012 RRID:SCR_002285
Biological sample
(Gallus gallus)
Hubbard JA57 strain Brüterei Stöckli, Ohmstal N/A