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. 2021 Mar 1;10:e62757. doi: 10.7554/eLife.62757

Figure 3. The mutation of ndrg1b affects gonadal development at 20 days post hatching (dph).

Figure 3.

Schematic representation of the experimental procedure adopted to analyze the effect of ndrg1b mutation on gonadal histology at 20 dph (D: dorsal, V: ventral) (A). Histological transverse sections of gonads. Wild-type (wt) = injected with cas9 or sgN1b=injected with cas9+sg1_ndrg1b from individuals for XX (B) and XY (C). In the testis, each cyst of germ cells (spermatogonia-like cells) is encircled by a black dotted line. Scale bars in B and C represent 50 µm. Total number of oogonia (D), number of pre-vitellogenic oocytes (PVO) (E), number of spermatogonial cysts (F), and number of spermatogonia per cyst (G) in wt female or male embryos (represented by gray empty bars or full bars, respectively) and sgN1b female or male embryos (represented by cyan empty bars or full bars, respectively). n = 16 per female groups and n = 20 per male groups. p-Values are indicated when the number of germ cells between groups differs significantly (p<0.05). NS: not statistically significant. Unpaired Student’s t-test.