Figure 6. Cell-type specific KI with TKIT.

(A) Schematic of experimental design for targeting excitatory neurons using Camk2a^Cre:Cas9^fl/wt animals (B) Super-resolution Airyscan confocal image of a CA1 pyramidal neuron with TKIT-mediated SEP-GluA1 KI illustrated as a maximum intensity projection. The CA1 cell layer is illustrated with a magenta dotted line with dendrites in Stratum radiatum. A zoomed in region (right) shows the dendritic segment indicated with a white box. (C) Schematic of experimental design for targeting PV positive interneurons using Pvalb^Cre/wt:Cas9^fl/wt animals. (D) Super-resolution Airyscan confocal image of a CA1 PV neuron with TKIT-mediated SEP-GluA1 KI illustrated as a maximum intensity projection. The CA1 cell layer is illustrated with a magenta dotted line with dendrites in Stratum radiatum. A zoomed in region (right) shows the dendritic segment indicated with a white box. (E) Schematic of experimental setup for two-photon image of virus-mediated TKIT GluA2 KI. (F) In vivo two-photon 3D reconstruction of a layer 2/3 pyramidal neuron in barrel cortex with TKIT-mediated SEP-GluA2 KI. High-magnification images of dendritic segments (right) from a maximum intensity projection of the same cell. Scale bars are indicated in the bottom right corner of images.