Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | GluA1 | GenBank; Ensembl | ID: 14799; ENSMUSG00000020524 | |
| Gene (Mus musculus) | GluA2 | GenBank; Ensembl | ID: 14800; ENSMUSG00000033981 | |
| Gene (Mus musculus) | GluA3 | GenBank; Ensembl | ID: 53623; ENSMUSG00000001986 | |
| Gene (Mus musculus) | GluN1 | GenBank; Ensembl | ID: 14810; ENSMUSG00000026959 | |
| Gene (Mus musculus) | GluN2A | GenBank; Ensembl | ID: 14811; ENSMUSG00000059003 | |
| Gene (Mus musculus) | GluN2B | GenBank; Ensembl | ID: 14812; ENSMUSG00000030209 | |
| Gene (Mus musculus) | Gephyrin | GenBank; Ensembl | ID: 268566; ENSMUSG00000047454 | |
| Gene (Mus musculus) | beta2 | GenBank; Ensembl | ID: 14401; ENSMUSG00000007653 | |
| Gene (Rattus norvegicus) | rGluA1 | GenBank | ID: 50592 | |
| Gene (Rattus norvegicus) | rGluA2 | GenBank | ID: 29627 | |
| Strain, strain background (Mus musculus, male and female) | Camk2a-Cre mouse | Jackson Laboratory strain 005359 | IMSR Cat# JAX:005359, RRID:IMSR_JAX:005359 | |
| Strain, strain background (Mus musculus, male and female) | Pvalb-Cre mouse | Jackson Laboratory strain 008069 | IMSR Cat# JAX:008069, RRID:IMSR_JAX:008069 | |
| Strain, strain background (Mus musculus, male and female) | Cas9fl/fl mouse | Jackson Laboratory strain 027632 | IMSR Cat# JAX:027632, RRID:IMSR_JAX:027632 | |
| Genetic reagent (Streptococcus pyogenes) | px330 | RRID:Addgene_42230 | RRID:Addgene_42230 | |
| Genetic reagent (Streptococcus pyogenes) | pAAV-nEF-SpCas9 | RRID:addgene_87115 | RRID:Addgene_87115 | |
| Genetic reagent (Escherichia coli) | pMiniT Vector | NEB | E1202 | NEB replaced this vector with pMiniT 2.0 Vector |
| Genetic reagent (Mus musculus) | AAV2/9-TKIT-SEP-GluA1 | Viral Core at the Janelia Research Campus | AAV for in vivo delivery TKITdonor and two guides | |
| Genetic reagent (Mus musculus) | AAV2/9-TKIT-SEP-GluA2 | Viral Core at the Janelia Research Campus | AAV for in vivo delivery TKITdonor and two guides | |
| Genetic reagent (Streptococcus pyogenes) | AAV2/9-nEF-HA-spCas9 | Viral Core at the Janelia Research Campus | AAV for in vivo delivery TKITdonor and two guides | |
| Genetic reagent (Aequorea victoria) | AAV2/9.CMV.PI.EGFP.WPRE.bGH | RRID:addgene_105530 | RRID:Addgene_105530 | |
| Biological sample (M. musculus) | Primary neuron culture | isolated from E18 mouse embryo and cultured | ||
| Biological sample (R. norvegicus) | Primary neuron culture | isolated from E18 rat embryo and cultured | ||
| Antibody | Anti-GluA1 (Rabbit polyclonal) | Homemade | JH4294 | IF (1:200) |
| Antibody | Anti-GluA2/3 (Rabbit polyclonal) | Homemade | JH4854 | IF (1:250) |
| Antibody | Anti-GluA2 (mouse monoclonal) | Eric Gouaux Lab | clone 15F1 | IF (1:5000) |
| Antibody | Anti-GluN1 (mouse monoclonal) | NeuroMab, clone N308/48 | UC Davis/NIH NeuroMab Facility Cat# N308/48, RRID:AB_2877408 | IF (1:200) |
| Antibody | Anti-GluN2a (Rabbit polyclonal) | Homemade | JH6097 | IF (1:200) |
| Antibody | Anti-GluN2b (Rabbit polyclonal) | Millipore, AB1548 | Millipore Cat# AB1548, RRID:AB_90759 | IF (1:100) |
| Antibody | Anti-beta2 (mouse monoclonal) | Millipore, MAB341 | Millipore Cat# MAB341, RRID:AB_2109419 | IF (1:200) |
| Antibody | Anti-PSD95 (mouse monoclonal) | NeuroMab, clone K28/43 | UC Davis/NIH NeuroMab Facility Cat# K28/43, RRID:AB_2877189 | IF (1:2500) |
| Antibody | Anti-Gephyrin (mouse monoclonal) | Synaptic systems, cat# 147011 | Synaptic Systems Cat# 147 011, RRID:AB_887717 | IF (1:2500) |
| Antibody | Anti-GFP (chicken polyclonal) | Abcam, cat # ab13970 | (Abcam Cat# ab13970, RRID:AB_300798) | IHC (1:5000) |
| Antibody | Anti-GFP (chicken polyclonal) | AVES, cat# GFP-1020 | Aves Labs Cat# GFP-1020, RRID:AB_10000240 | IF (1:5000) |
| Antibody | Anti-myc (mouse monoclonal) | Homemade | Clone 9E10 | IF (1:1000) |
| Antibody | Anti-HA (Rabbit monoclonal) | Cell Signaling, clone C29F4 | Cell Signaling Technology Cat# 3724, RRID:AB_1549585 | IF (1:500) |
| Recombinant DNA reagent (Escherichia coli) |
pSG | This paper | For the guide2 cloning | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
GluA1 (px330:2-guide-Cas9) | This paper | Twoguides for GluA1 (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-GluA1 TKIT donor | This paper | TKIT donor for SEP-GluA1 (mouse) in pMiniT | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:myc-GluA1 TKIT donor | This paper | TKIT donor for myc-GluA1 (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
GluA2 (px330:2-guide-Cas9) | This paper | Two guides for GluA2 (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-GluA2 TKIT donor | This paper | TKIT donor for SEP-GluA2 (mouse) in pMiniT | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:tdTomato-GluA2 TKIT donor | This paper | TKIT donor for tdTomato-GluA2 (mouse) in pMiniT | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:myc-GluA2 TKIT donor | This paper | TKIT donor for myc-GluA2 (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
GluA3 (px330:2-guide-Cas9) | This paper | Two guides for GluA3 (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-GluA3 TKIT donor | This paper | TKIT donor for SEP-GluA3 (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
GluN1 (px330:2-guide-Cas9) | This paper | Two guides for GluN1 (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-GluN1 TKIT donor | This paper | TKIT donor for SEP-GluN1 (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
GluN2A (px330:2-guide-Cas9) | This paper | Two guides for GluN2A (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-GluN2A TKIT donor | This paper | TKIT donor for SEP-GluN2A (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
GluN2B (px330:2-guide-Cas9) | This paper | Two guides for GluN2B (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-GluN2B TKIT donor | This paper | TKIT donor for SEP-GluN2B (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
Gephyrin (px330:2-guide-Cas9) | This paper | Two guides for Gephyrin (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:GFP-Gephyrin TKIT donor | This paper | TKIT donor for GFP-Gephyrin (mouse) in pMiniT | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:tdTomato-Gephyrin TKIT donor | This paper | TKIT donor for tdTomato-Gephyrin (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
beta2 (px330:2-guide-Cas9) | This paper | Two guides for beta2 (mouse) in px330 | |
| Recombinant DNA reagent (Mus musculus) |
pMiniT:SEP-beta2 TKIT donor | This paper | TKIT donor for SEP-beta2 (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
rGluA1 (px330:2-guide-Cas9) | This paper | Two guides for rGluA1 (mouse) in px330 | |
| Recombinant DNA reagent (Rattus norvegicus) |
pMiniT:SEP-rGluA1 TKIT donor | This paper | TKIT donor for SEP-rGluA1 (mouse) in pMiniT | |
| Recombinant DNA reagent (Streptococcus pyogenes) |
rGluA2 (px330:2-guide-Cas9) | This paper | Two guides for rGluA2 (mouse) in px330 | |
| Recombinant DNA reagent (Rattus norvegicus) |
pMiniT:SEP-rGluA2 TKIT donor | This paper | TKIT donor for SEP-rGluA2 (mouse) in pMiniT | |
| Recombinant DNA reagent (Mus musculus) |
pAAV-SEP-GluA1 TKIT | This paper | TKIT donor and two guides for SEP-GluA1 (mouse) in one pAAV vector | |
| Recombinant DNA reagent (Mus musculus) |
pAAV-SEP-GluA2 TKIT | This paper | TKIT donor and two guides for SEP-GluA2 (mouse) in one pAAV vector | |
| Commercial assay or kit | NEBuilder HiFi DNA Assembly Cloning Kit | NEB | cat#: E5520 | |
| Software, algorithm | Fiji/ImageJ | Fiji | Fiji, RRID:SCR_002285 | |
| Software, algorithm | GraphPad Prism 7.0 | GraphPad Software | GraphPad Prism, RRID:SCR_002798 | |
| Software, algorithm | Benchling CRISPR tool | http://www.benchling.com | Benchling, RRID:SCR_013955 | |
| Software, algorithm | IMARIS 9.6.0 (Bitplane) | BitPlane | Imaris, RRID:SCR_007370 | |
| Other | Prolong Glass Antifade mounting media | Thermo Scientific | P36984 |