Figure 2:

Histology-based microstructural analysis of right ventricular myocardium. (A) We divided each sample into two parts along a plane normal to the fiber direction. We sectioned the first part in ten 1mm-steps in the transmural direction, and we sectioned the second part in ten 1mm-steps along the basal-apical direction, i.e, longitudinally. (B) Representative example of a histological section stained with Masson’s trichrome. (C) Identification of fiber orientation distributions via ImageJ’s OrientationJ plugin, where the color scale represents the in-plane fiber angle with respect to the horizontal axis. (D) We also fit π-periodic von Mises probability density functions (PDF) to the OrientationJ-derived normalized pixel occurrence per fiber angle for each section in both sample parts