Mechanics of right ventricular myocardium can be accurately predicted based on microstructural data. Shown are raw data for a selected sample, which also serves as the “test data” (black circles). The red line shows a direct, or optimal, constitutive fit via Strategy 4, that is using the Holzapfel model that accounts for spatial variation in histology-informed fiber dispersion through the sample’s thickness. Note, this fit quality is typical in that it falls between the best and worst fits. We also show a prediction based on constitutive parameters that were informed via training on the other ten samples and the microstructural data from the sample itself