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. 2021 Feb 18;40(10):1896–1908. doi: 10.1038/s41388-021-01686-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Dual-luciferase assay: luciferase was expressed from the SV40 promoter and used to normalise the Renilla expression under the control of the putative promoter fragments. Their location relative to the gene structure is depicted. Values represent mean ± standard deviation, six transfections per construct. Promoterless luciferase vector was used as a negative control. bmMSC bone marrow mesenchymal stem cells, osb porcine flTP53^R167H/R167H osteoblasts, OS porcine flTP53^R167H/R167H osteosarcoma cells, KDNF porcine kidney fibroblasts, HEK293 human embryonic kidney cell line. b Quantitative PCR results of Δ152p53α mRNA expression in OS (n = 48) and matched healthy bone samples. c Representative western blots showing Δ152p53α protein and MDM2 expression in OS and healthy matched bone samples of homozygous flTP53^R167H pigs. d Quantitative measurements of proteins in OS (n = 10) and healthy matched bone samples from homozygous flTP53^R167H pigs. e The Δ152p53α mRNA expression in small (<5 cm, n = 29) vs. large (>5 cm, n = 19) tumours. f Age-dependent Δ152p53α mRNA expression in healthy bones of homozygous flTP53^R167H (n = 10) and wild-type (n = 3) pigs. g Western blots showing Δ152p53α isoform expression kidney and spleen tumours and healthy matched tissues of flTP5^R167H/R167H pigs. h QPCR analysis of Δ152p53α mRNA expression in blood exosomes from flTP53^R167H heterozygous (n = 6), homozygous (n = 6) and wild-type (n = 3) pigs aged 3 and 10 months. i Migration and invasion Transwell assays for pig OS cells transfected with mutant R167H Δ152p53α isoform or a GFP control vector. Left, representative microscopic image (scale bars, 200 μm). Right, quantification of the indicated migrated and invaded cells numbers.