Skip to main content
. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Feb 10;206(6):1329–1336. doi: 10.4049/jimmunol.2000969

Figure 2. Inflammasome activation is decreased in ABL KO THP-1 cells.

Figure 2.

Two stable CRISPR/Cas9 KO THP-1 strains immunoblotted for cAbl kinase and actin proteins are shown (A). The effect of the cAbl kinase deletion on inflammasome activation after LPS/ATP stimulation (3h+30min) is shown in (B) and after LPS stimulation for 16h in (C). IL-1β release was used as a measure of inflammasome activation. The effect of cAbl depletion on LDH release after rapid inflammasome activation is shown in (D). IL-18 release was measured by ELISA after rapid inflammasome activation by LPS for 30 min followed by ATP for 30 min (E) or nigericin (F). n=7–10 independent experiments. *** p<0.001, ** p<0.01, * p<0.05 as compared to Cas9 control cells.