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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Feb 10;206(6):1329–1336. doi: 10.4049/jimmunol.2000969

Figure 5. ASC phosphorylation is reduced in released ASC dimers and trimers from ABL KO THP-1 cells.

Figure 5.

ASC oligomerization and phosphorylation were compared between THP-1 cells, ABL KO THP-1 cells and ASC KO THP-1 cells by comparing the monoclonal anti-Y144 ASC antibody to the polyclonal ASC antibody after LPS+ATP stimulation (30 min+30 min) (A). Tyrosine phosphorylation of released ASC in the modified THP-1 cells after LPS+ATP (30 min+30 min) was imaged with Y144 antibody (B). After Y146 imaging, blots were stripped and re-blotted with the polyclonal ASC antibody. Arrows show ASC monomer (22 kDa), and dimer (44 kDa) and line shows oligomers (66 kDa and up). Densitometry data are expressed as a ratio of intensity of P-tyrosine bands to the corresponding ASC bands.