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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Jan 27;206(6):1385–1394. doi: 10.4049/jimmunol.2000792

FIGURE 4.

FIGURE 4.

CD155 and PD-L1 are induced in Th1 and Th2 contexts in murine splenic macrophages. (A-D) CD155 and PD-L1 expression, and co-expression of both, were measured by flow cytometry on murine splenic macrophages after mock (PBS), CFA (Th1) or IFA (Th2) immunization with OVA challenge. Panel (C) shows isotype controls and representative flow cytometry results from one animal in each of the experimental groups. Panel (D) shows that PD-L1 induction only occurred on macrophages with elevated CD155 expression. (N=4 for PBS and IFA; n=3 for CFA). (E) The mPVR region upstream of the transcriptional start site contains a DRE identified as an AhR responsive site by JASPAR analysis (compare to PVR in Figure 3A). (F) Mice were treated with vehicle control or SR1 48hrs and 1h before IFA immunization or PBS treatment, and 48hrs and 1h before PBS (mock) immunization or OVA challenge. CD155 and PD-L1 expression was measured as in (A-C). (N=9 for PBS and IFA; n=10 for IFA+SR1).