Figure 4. MiR-210 mimic downregulated RyR2 and Ca²⁺ sparks in uterine arteries.

A. RyR2 is a bona fide target of miR-210 in uterine arteries. Schematic representation of alignment of the predicted miR-210 binding site to ovine RyR2 3′UTR is shown. Uterine arterial smooth muscle cells were treated with 100 nmol/L miR-210 mimic (miR-210) or negative control (Neg Ctr) for 24 hours and RISC-IP analysis of abundance of RyR2 3′UTR pulled down by Ago1/2/3 antibody was determined after the treatment. B - D. Uterine arteries from normoxic control pregnant sheep were treated with 100 nmol/L miR-210 or Neg Ctr under 21.0% O₂ for 48 hours. RyR2 protein abundance (B), the percentage of myocytes with Ca²⁺ sparks (C) and Ca²⁺ spark frequency (sparks/μm/s) (D) were determined after the treatment. Data are means ± SEM of 5 animals in each group; independent-samples t-test; *P < 0.05, miR-210 versus Neg Ctr.