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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Feb 8;206(6):1361–1371. doi: 10.4049/jimmunol.2000973

Figure 5. IFNγ is produced by circulating, milk-activated pediatric EoE T cells.

Figure 5.

A) Flow-cytometric analysis of CD4+ T cells from peripheral blood of non-EoE (control) or milk-allergic EoE (EoE) pediatric subjects. PBMCs were cultured in vitro in the absence (Unstimulated; Unstim.) or presence of tetanus toxoid or milk proteins (α-lactalbumin, β-lactoglobulin, and α/β/κ-casein) for 6 days. Gated on Live, CD8−, CD19−, CD4+. Numbers indicate percentage of parent gate. B) IFNγ production by peripheral CD4+ T cells across multiple non-EoE or milk-allergic EoE subjects. C) IFNγ amounts in culture supernatants of peripheral CD4+ T cells from non-EoE or milk-allergic EoE subjects cultured in vitro in the presence of milk proteins. N=8-9 subjects per experimental arm; *P≤0.05.