Table 2. Mass Spectrometry Analysis of Selected Arachidonic Acid Metabolites.
Aorta samples were harvested at the end of 4 weeks and were cleaned free of peri-adventitial fat. Selected vasoactive metabolites of arachidonic acid were detected by LC-MS/MS analysis (pg/mg protein, n=6). Data are presented as mean ± SEM. The effects of genotype, diet and interaction in two-way ANOVA are indicated by Pgenotype, Pdiet and Pinteraction. n.s. signifies not significant; N.D. signifies not detectable and zero value was entered for data analysis.
| Metabolite | Two-way ANOVA | NT Chow | NT HSD | S-P467L Chow | S-P467L HSD | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Pgenotype | Pdiet | Pinteraction | Mean | S.E. | Mean | S.E. | Mean | S.E. | Mean | S.E. | |
| PGE2 | 0.15 | 0.09 | 0.10 | 776.3 | 157.3 | 776.9 | 203.6 | 690.7 | 161.9 | 1891.3 | 613.4 |
| PGD2 | 0.17 | 0.13 | 0.11 | 760.6 | 168.5 | 731.5 | 230.6 | 684.3 | 164.7 | 1840.9 | 637.4 |
| PGF2α | 0.25 | 0.12 | 0.15 | 271.2 | 63.8 | 299.5 | 87.4 | 221.6 | 47.4 | 759.4 | 322.8 |
| 8-Iso PGF2α | 0.52 | 0.52 | 0.11 | 35.3 | 26.4 | N.D. | N.D. | 15.2 | 15.2 | ||
| LTB4 | 0.15 | 0.69 | 0.96 | 202.8 | 10.9 | 197.0 | 12.9 | 183.7 | 17.4 | 179.2 | 3.6 |