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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Feb 5;206(6):1395–1404. doi: 10.4049/jimmunol.2000500

Figure 2. Development of ASAH2-selective small molecule inhibitor NC06.

Figure 2.

A. NC06 chemical structure. B. RBM14C16 was used as substrate and incubated with recombinant human (top panel) and mouse (bottom panel) Asah2 proteins in the presence of NC06 at the indicated concentrations. The IC50 was determined using GraphPad Prism. The enzyme activity assays were performed twice.