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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Jan 29;206(6):1171–1180. doi: 10.4049/jimmunol.2001131

Figure 4: NK cells produce IL-10 in an IL-15-dependent manner.

Figure 4:

(A) Experimental Design: Splenocytes were harvested from IL-10bit mice, cells express Thy1.1 when IL-10 is expressed. IL-10bit splenocytes were then stimulated with either 1 or 104 pg/mL of IL-15 for either 24 or 48hrs. BfA was added during the last 4hrs of stimulation (either 20 or 44hrs, respectively) and the frequency of IFNγ and IL-10 expressing NK cells (NK1.1+CD3−) was determined by ICS. (B) Representative Staining: NK cells expressing Thy1.1 (IL-10) and IFNγ at 24 or 48hrs post stimulation with either 1 or 104 pg/mL IL-15. Frequency of (C) IFNγ- and (D) IL-10-expressing NK at 24 or 48hrs post stimulation with either 1 or 104 pg/mL of IL-15. Data are representative from 2 independent experiments with at least 4–5 mice per group. * = p<0.05 for 24hrs #=p<0.05 for 48 hrs. Error bars represent standard error of the mean.