Table 2.
Gene expression in whole placenta and isolated labyrinth
| ddPCR | Gene | ND | WD | P-value |
|---|---|---|---|---|
| Labyrinth | Lpcat1 | 43.41 ± 15.39 | 17.89 ± 6.22 | 0.16 |
| Lpcat2 | 10.02 ± 4.76 | 11.47 ± 1.91 | 0.91 | |
| Cept | 8.41 ± 3.57 | 6.45 ± 2.97 | 0.70 | |
| Pcyt1a | 38.83 ± 17.09 | 30.64 ± 8.35 | 0.67 | |
| Pla2g7 | 125.70 ± 46.96 | 53.53 ± 16.23 | 0.22 | |
| Elovl1 | 401.15 ± 114.58 | 202.95 ± 56.96 | 0.16 | |
| Fads1 | 129.53 ± 52.33 | 88.43 ± 15.50 | 0.59 | |
| Fads2 | 248.20 ± 98.8 | 112.00 ± 25.86 | 0.27 | |
| Pemt | 8.00 ± 2.03 | 4.66 ± 1.68 | 0.30 | |
| Whole Placenta | Lpcat1 | 82.78 ± 36.43 | 30.36 ± 16.3 | 0.23 |
| Lpcat2 | 13.04 ± 3.71 | 8.21 ± 3.00 | 0.36 | |
| Cept | 19.04 ± 6.322 | 8.07 ± 1.26 | 0.13 | |
| Pcyt1a | 4.63 ± 1.97 | 3.91 ± 0.85 | 0.75 | |
| Pla2g5 | 1.59 ± 0.47 | 1.94 ± 0.53 | 0.64 | |
| Pla2g7 | 126.33 ± 23.44 | 128.30 ± 13.05 | 0.94 | |
| nCounter | Gene | WD/ND | P-value | |
| Labyrinth | Apoam | 20.00 | 0.06 | |
| Apoa2 | 8.58 | 0.08 | ||
| Apoa1 | 12.70 | 0.09 | ||
| Apoc2 | 7.76 | 0.26 | ||
| Apoe | 4.37 | 0.01 | ||
| Apoa4 | 15.8 | 0.04 | ||
| Apob | 19.4 | 0.05 | ||
| Slc6a12 | 1.24 | 0.66 | ||
| Slc16a6 | 0.94 | 0.69 | ||
| Slc1a5 | 1.04 | 1.00 | ||
| Slc27a1 | 0.99 | 1.00 | ||
| Slc3a2 | 1.07 | 0.24 | ||
| Slc7a5 | 0.90 | 0.57 | ||
| Acox1 | 1.17 | 0.04 | ||
| Mcat | 0.82 | 0.16 | ||
| Slc25a1 | 1.20 | 0.42 | ||
| Fabp5 | 0.91 | 0.78 | ||
| Slc2a3 | 1.49 | 0.25 | ||
| Slc2a1 | 0.98 | 0.91 |
ddPCR, copies of target gene/10,000 copies Actb ± SEM normalized are shown in ND and WD samples nCounter, ratio of WD to ND counts (WD/ND) of representative genes from the nCounter Metabolism Panel.