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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Jan 29;206(6):1297–1314. doi: 10.4049/jimmunol.2000965

FIGURE 6.

FIGURE 6.

Adoptive transfer of moDCs reconstitutes chronic lung disease in Tlr3–/– mice. (a) Protocol scheme for isolation of moDCs from lungs of WT mice, then transfer (1×106 cells) into Tlr3–/– mice at 1 d before SeV, and then assay for disease at 49 d after SeV infection. (b) PAS and hematoxylin staining of lung sections for indicated cell transfer conditions at 49 d after infection with SeV. Small scale bar, 500 μm; large scale bar, 250 μm. (c) Lung sections from (b) with hematoxylin and PAS staining colorized red with image analysis. (d) Muc5ac immunostaining of lungs for conditions in (b). Small scale bar, 500 μm; large scale bar, 250 μm. (e) Quantification of red colorized staining from (c) and immunostaining from (d). All data are representative of three separate experiments (mean and SEM) with at least 5 mice per condition in each experiment. *P<0.01.