FIGURE 8.

Adoptive transfer of BMDCs causes minor lung disease without viral infection in Tlr3^–/– mice. (a) Protocol scheme for isolation and transfer of BMDCs from WT into Tlr3^–/– mice. (b) Histograms from flow cytometry analysis of CD11c⁺ and CD11b⁺ cells in BMDC cultures for conditions in (a). (c) Histograms for intracellular TLR3 in BMDCs from WT or Tlr3^–/– mice for conditions in (b). (d) Immunostaining for Sftpc and GFP and counterstaining for DAPI of lung sections from Tlr3^–/– mice at 1 d after transfer of BMDCs from Tlr3^–/– or CAG-gfp mice (0 d after SeV infection). Large scale bar, 100 μm; small scale bar, 10 μm. (e) PAS and hematoxylin staining of lung sections for indicated cell transfer conditions at 21 d after PBS. Small scale bar, 500 μm; large scale bar, 250 μm. (f) Lung sections from (e) with hematoxylin and PAS staining colorized red with image analysis. (g) Quantification of colorized staining from (f). All data are representative of three separate experiments (mean and SEM) with at least 5 mice per condition in each experiment. *P<0.01.