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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: J Immunol. 2021 Jan 29;206(6):1297–1314. doi: 10.4049/jimmunol.2000965

FIGURE 9.

FIGURE 9.

Adoptive transfer of BMDCs reconstitutes chronic lung disease after viral infection in Tlr3–/– mice. (a) Protocol scheme for isolation and transfer of BMDCs from WT into Tlr3–/– mice. (b) Immunostaining for Sftpc and GFP and counterstaining for DAPI of lung sections from Tlr3–/– mice after transfer of BMDCs from Tlr3–/– or CAG-gfp mice and then 0–12 d after SeV infection. Scale bar, 100 μm. (c) Quantification of GFP+ immunostaining from (b). (d) PAS and hematoxylin staining of lung sections for indicated cell transfer conditions at 49 d after infection with SeV. Small scale bar, 500 μm; large scale bar, 250 μm. (e) Lung sections from (c) with hematoxylin and PAS staining colorized red with image analysis. (f) Quantification of colorized staining from (d). All data are representative of three separate experiments (mean and SEM) with at least 5 mice per condition in each experiment. *P<0.01.