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. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: Nat Genet. 2021 Feb 11;53(3):367–378. doi: 10.1038/s41588-021-00784-4

Extended Data Fig. 3: Experimental protocol and computational workflow for DpnII-seq.

Extended Data Fig. 3:

(A) Schematic of DpnII-seq experimental protocol for recovering DNA fragments digested by the restriction enzyme DpnII.

(B) Directed graph of DpnII-seq computational pipeline

(C) Histogram of distance to nearest DpnII recognition site for each recovered DpnII digested fragment.

(D) Raw DpnII-seq signal displaying multiple copy number states (2N, 3N, 4N) within chromosome 3 (data binned at 40 kb).

(E) Copy number corrected DpnII-seq signal displaying single copy number state (2N) across chromosome 3.