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. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: Nat Genet. 2021 Feb 11;53(3):367–378. doi: 10.1038/s41588-021-00784-4

Fig. 7: Illustration of chromatin interaction dynamics in the nucleus and model for cohesin loss after chromatin digestion.

Fig. 7:

(A) Left: Schematic representation of varying chromatin interactions dynamics at different sub-nuclear domains. Shortest half-life reflects the least stable interactions (yellow), while longest half-life reflects the most stable interactions (dark orange). Nuclear subdomains differ greatly in their stability. Top right: Chromatin anchored at speckles is driven by the most dynamic interactions. Bottom right: Chromatin anchored at the nuclear lamina involves the most stable interactions.

(B) Model for how cohesin rings stabilize CTCF-CTCF loops by encircling loop bases. Top: Cohesin ring encircles loop bases at convergent CTCF sites. Middle: Pre-digestion with DpnII cuts loop into chromatin fragments <6 kb, and the cohesin ring can slide off nearby ends. Bottom: CTCF remains bound to digested chromatin fragments but interactions between CTCF-bound sites are lost.