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. 2021 Feb 25;12:607044. doi: 10.3389/fimmu.2021.607044

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Copyright © 2021 Renrick, Thounaojam, de Aquino, Chaudhuri, Pandhare, Dash and Shanker

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time kinetics shows the increase of miR-155 and the decrease of its targets suppressor of cytokine signaling 1 (SOCS1) and SH-2 containing inositol 5’-polyphosphatase 1 (SHIP1) in T1 cells following bortezomib treatment. Human lymphoblast T1 cells were treated in vitro with 10 nM bortezomib for 2, 4, 6, 9, 12, 18, and 24 h. The cells were collected at each time point and analyzed for miR-155 expression by RT-PCR and for SOCS1 and SHIP1 protein levels by Western blotting. The expression of miR-155 (A) and SOCS1 and SHIP1 protein levels (B) are shown by function of time in T1 cells upon 10 nM bortezomib treatment. Numbers above the bands are the indicated ratios of the densitometry values. (C) Bar graphs depict SHIP1:β-actin ratios for fold changes in SHIP1 protein as calculated from the densitometry values. Data are presented as mean values ± SD from four independent experiments with triplicates; ^* p < 0.0072, ^** p < 0.0001, ^*** p < 0.00001 (ANOVA, one-way), compared to 0 h (A, C).