Table 2.
Primer sequence for reference genes and genes of interest and qPCR efficiency.
| Gene symbol | Primers 5′-3′ forward, reverse | Gene ID | Amplicon bp | E % | R2 |
|---|---|---|---|---|---|
| Reference genes | |||||
| ACTB | CCTGAACCCCAAGGCCAACCG GGCGTACAGGGACAGCACGG | XM_003357928 | 106 | 97.8 | 0.999 |
| B2M | CAACCACTTTTCACACCGCTCCAGT ACCGCATCCAGGCCAGACAGT | NM_213978 | 91 | 99.4 | 1.000 |
| GAPDH | ACACTCACTCTTCTACCTTTG CAAATTCATTGTCGTACCAG | DQ_845173 | 90 | 102.0 | 1.000 |
| HPRT | GCAGCCCCAGCGTCGTGATTAG AGCAAGCCGTTCAGTCCTGTCC | NM_001032376 | 140 | 99.0 | 1.000 |
| SDHA | GTGGTGGTTGGTGCCGGAGG GCCTCCCTGGGCTGCAACAG | XM_003362140 | 126 | 101.2 | 0.998 |
| RPL4 | CCGCGCACCACGCAAGAAGA CCTGGCCTGGCGAAGAATGGT | DQ_845176 | 130 | 97.0 | 1.000 |
| Genes of interest | |||||
| IL1β | ACCCGCCAAGGAAGTGATGGC GGTCCAGGTTTTGGGTGCAGCA | NM_214055 | 104 | 96.0 | 0.999 |
| ICAM1 | GCTCACCCACCGCTGCATGT CGGAACCGGGCATCTGTCGG | NM_213816 | 123 | 97.6 | 0.998 |
| IL18 | ATGGCTGCTGAACCGGAAGAC ACACGGCTTGATGTCCCTGGT | NM_213997 | 193 | 99.4 | 0.999 |
| IL8 | GGTCTGCCTGGACCCCAAGGA TGGGAGCCACGGAGAATGGGT | NM_213867 | 124 | 97.2 | 1.000 |
| IL10 | GAGCTGCCTTCGGCCCAGTGA ACAAGGCTTGGCAACCCAGGTA | NM_214041 | 117 | 100.2 | 1.000 |
| TNF | GCCCGTCGCCCACGTTGTAG CTTCACGCCGTTGGCCAGGA | NM_214022 | 94 | 102.6 | 1.000 |
| IL1RN | GTGTCCTGTTGTTGCATGGTC AGGTCTCTTTCCCAAGGGGT | NM_214262 | 123 | 97.9 | 0.998 |
| HSP90 | TCCCCAGACGCACGCCAACA AGGGGTGGCATCTCCTCCGT | NM_213973 | 117 | 98 | 1.000 |
| NFKB1 | CACTCGCTGCCCTTCTCGCC AAAGGACGTCTCCACGCCGC | NM_001048232 | 93 | 99.0 | 1.000 |
| STAT3 | CCGGCCCATGCTGGAGGAGA GCCGGTCAGGGTGCATAGGC | NM_001044580 | 104 | 99.8 | 1.000 |
Amplicon bp, amplicon length, base pairs; E, efficiency of assay; R2, correlation coefficient.