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. 2021 Feb 25;12:618610. doi: 10.3389/fimmu.2021.618610

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Copyright © 2021 Lee, Alrasheed, Khandelwal, Fitzsimons, Richards, Wilson, Chavda, Henry, Conde, De Massy, Chin, Galas-Filipowicz, Herrero, Chain, Quezada and Yong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of T cell subsets by flow cytometry of paired BM samples at diagnosis and D100 post-ASCT. (A) Change in frequency of total CD3, (B) CD4, (C) CD8, (D) CD4:CD8 ratio, (E) Tregs, (F) CD8:Treg ratio (G) CD4:Treg ratio. Ratios calculated using absolute numbers of populations in each sample. (H) Changes in CD4eff memory subtypes as defined by CCR7 and CD45RA expression, (I) CD8 memory subtypes. Statistical analysis by paired Student’s t-tests *p < 0.05, ***p < 0.001, ****p < 0.0001.